Fig. 1

CT and immunohistochemistry of the right adrenal lesion. a Abdominal CT image showing a right adrenal lesion (arrow). b Gross appearance of the resected right adrenal gland, and immunohistochemical double-staining for SF-1 and CGA: the adrenal capsule (ac), residual normal adrenal cortex (nac; stained brown for SF-1), tumour capsule (tc), cortical component of the tumour (cct; stained brown for SF-1), and medullary component of the tumour (mct; stained red for CGA) are visible (×200). c-h Immunohistochemical staining for the cancer stem cell-specific markers ALDH1 (c), CD44 (d), CD133 (e), Nestin (f), NGFR (g), and SOX9 (h) (×400 and × 1000)