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Table 2 Sequences of oligonucleotide primers used for PCR amplification and minigene construction

From: Splicing analysis of CYP11B1 mutation in a family affected with 11β-hydroxylase deficiency: case report

Primer

Sense Strand

Antisense Strand

CYP11B1_Exon 1

5’- GTTCTCCCATGACGTGATCCCTCT – 3’

5’- TCCAAAGGATGCAGAGTGCC – 3’

CYP11B1_Exon 2

5’ – TGGACAGGAGACACTTTGGAT – 3’

5’ – TCGCCGCTTACAGCAAGAAC – 3’

CYP11B1_Exon 3–4

5’ – TGGGGACAAGGAGGATGGGATAC – 3’

5’ – TGGTGGAGAGGGAGAAATTGGG – 3’

CYP11B1_Exon 4

5’ – CGTGGGAAGATCCAGCCTCAG – 3’

5’ – GGAAGGTGAGGAATCCCCGAC – 3’

CYP11B1_Exon 5

5’ – AGGAGGAGGACACTGAAGGATG – 3’

5’ – AGGCAGGCTTGGCATCACC – 3’

CYP11B1_Exon 6

5’ – GGCTCTGTCGTTCTCAGGGTATGC – 3’

5’ – GGCGTTGAAGAGGGATTCCAGAG – 3’

CYP11B1_Exon 7–8

CYP11B1_Exon 9

CYP11B1_IVS7_construct

5’ – AGAGAGCACAGGAAGCCCCATC – 3’

5’ – GTTCCCCCTTCAGCATAATCTC – 3’

5’ - AGTCGGATCCCTTGCTGATGACGCTCTTTG - 3’

5’ – CAGTCCCACATTGCTCAAGC – 3’

5’ – GCCCTCGGGAGTTCCATTT – 3’

5’- GTACTCTAGAATGGCTCTGAAGGTGAGGAG - 3’