Fig. 1

Analysis of ACTG2 protein expression in SI-NETs by immunohistochemistry (a-d) using ACTG2 antibody (NB100-91649 Novus Biologicals) and western blotting (e) using another ACTG2 antibody (TA313418 Origene). a Negatively stained tumor cells and strong stromal staining (20x). b Areas with positively stained tumor cells, and negative stromal staining (20x). c Weak staining in all tumor cells (20x). d No staining in absence of primary antibody (20x). e Western blotting analysis showing antibody specificity and correlation to immunohistochemistry analysis. One band only was visualized in two tumors (lanes 2 and 3) displaying strong stromal staining, and no band was detected in two tumors (lanes 1 and 4) with no staining in both tumor and stromal cells. Lane 1, lymph node metastasis; lanes 2–4, primary tumors. Coomassie blue staining was used as loading control, ladder in kDa