Table 2 Primers used for RET amplification and sequencing
Oligo Name | Sequence (5′ → 3′) | Annealing Temperature (Ta) [°C] |
|---|---|---|
RET-2 F | GCT TCC CCT GTT TCC TTT TC | |
RET-2R | AGT GTC AGC GGC TGT GAT AA | |
RET-5 F | CGT GCA GCA TTC TAA GGT CTC | |
RET-5R | CAT GTC TGT AGG GTG CTG CT | |
RET-8 F | TGC TCC TGG CAC TGT CTT | |
RET-8R | TGG GGA CCA ATC ACT GTA CTC | |
RET-10 F | GGA CAC TGC CCT GGA AAT A | |
RET-10R | ACT CGC CTC CCA GCA ATT T | |
RET-11 F | ATA CGC AGC CTG TAC CCA CT | |
RET-11R | AAA TGG GGG CAG AAC ACA | 58 |
RET-13 F | CCA TCC TGA CCT GGT ATG GT | |
RET-13R | AAA CAG GGC AGG AGC AGT AG | |
RET-14 F | GTC CAC CCC CTT ACT CAT TG | |
RET-14R | GTG GTG AGC CAT AGC ATG G | |
RET-15 F | CCC CCG GCC CAG GTC TC | |
RET-15R | GCT CCA CTA ATC TTC GGT ATC TTT | |
RET-16 F | TCT CCT TTA CCC CTC CTT CC | |
RET-16R | CAG TGA GGG GGT CAT TGC |