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Figure 4 | BMC Endocrine Disorders

Figure 4

From: AMPD1 regulates mTORC1-p70 S6 kinase axis in the control of insulin sensitivity in skeletal muscle

Figure 4

Promoted mTORC1 formation in skeletal muscle after high fat diet feeding by AMPD1 deficiency. (A) Protein extracts from gastrocnemius muscle of wild type and AMPD1 deficient mice fed with normal chow and high fat diet were subjected to immunoblotting for mTOR, Raptor and Rictor (n = 5 for each group). The band intensity was quantified and adjusted with that of α-tubulin. Representative immunoblot images are shown in upper panels. Data shown in lower panels are mean ± SD expressed relative to that of WT mice fed with normal chow. (B) mTOR immunoprecipitates from gastrocnemius muscle were analyzed by immunoblotting with anti-Raptor antibody. The band intensity of mTOR-bound Raptor in immunoprecipitates was adjusted with that of α-tubulin in the input lysates used for immunoprecipitation. Representative immunoblot images of Raptor in mTOR immunoporecipitates and α-tubulin in the input lysates are shown in upper panels. Data shown in lower panel are mean ± SD expressed relative to that of WT mice fed with normal chow (n = 5 for each group). *indicates p < 0.05 vs. WT mice fed with high fat diet.

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