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Figure 2 | BMC Endocrine Disorders

Figure 2

From: AMPD1 regulates mTORC1-p70 S6 kinase axis in the control of insulin sensitivity in skeletal muscle

Figure 2

AMPD1 deficiency did not change phosphorylation levels of AMPK, Akt and p70 S6 kinase after high fat diet challenge in liver. Protein extracts from liver of wild type and AMPD1 deficient mice fed with normal chow and high fat diet were studied by immunoblotting for AMPK/phosphorylated AMPK (pAMPK), Akt/phosphorylated Akt (pAkt) and p70 S6 kinase/phosphorylated p70 S6 kinase (pp70 S6kinase) (n = 5 for each group). The ratio of the band intensity for phosphorylated form to that of total form was quantified and adjusted with that of α-tubulin. Representative immunoblot images are shown in upper panels. Data shown in lower panels are mean ± SD expressed relative to that of WT mice fed with normal chow.

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