Figure 3

Diabetes-induced upregulation of renal Complex-III subunits, Rieske protein and Core 2 protein. A) Coomassie blue stained SDS-Polyacrylamide gel (following immunoprecipitation of Complex-III in control (C) and diabetic (D) rat kidney mitochondria) revealing upregulation of mitochondrial Complex-III subunit proteins during diabetes. B) Complex III IP followed by Western blot analysis revealed upregulation of Complex III associated Rieske Protein and Core 2 Protein subunits during diabetes. C) Densitometric analysis showing Complex III associated subunits were upregulated during diabetes. D) Rieske Protein and Core 2 Protein Western blot analysis of total mitochondria. GAPDH Western blot analysis was used as a loading control. E) Densitometric analysis of the Western blot shown in panel D. Blots are representative of three separate experiments.