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Table 5 Relative mRNA expression of selected genes in MC3T3-E1 cells

From: Different skeletal effects of the peroxisome proliferator activated receptor (PPAR)α agonist fenofibrate and the PPARγ agonist pioglitazone

DAY   Alcalic phosphatase (ALP) Bone Sialoprotein CD44 Collagen 1 Lipoprotein lipase (LPL)
0   1.00 ± 0.05 1.00 ± 0.44 1.00 ± 0.30 1.00 ± 0.10 No detectable expression
4 Control 1.00 ± 0.15 1.00 ± 0.18 1.00 ± 0.03 1.00 ± 0.24 1.00 ± 0.51
  Fenofibrate 0.1 μM 0.62 ± 0.11 0.88 ± 0.23 1.05 ± 0.17 0.92 ± 0.18 1.12 ± 0.23
  Pioglitazone 0.1 μM 1.06 ± 0.19 1.40 ± 0.62 1.52 ± 0.06 1.17 ± 0.19 0.90 ± 0.01
8 Control 1.00 ± 0.27 1.00 ± 0.54 1.00 ± 0.17 1.00 ± 0.03 1.00 ± 0.12
  Fenofibrate 0.1 μM 1.47 ± 0.04 2.19 ± 0.24 1.09 ± 0.06 0.76 ± 0.0 0.81 ± 0.01
  Pioglitazone 0.1 μM 1.40 ± 0.16 2.07 ± 1.45 0.81 ± 0.04 0.34 ± 0.04 1.00 ± 0.06
12 Control 1.00 ± 0.12 1.00 ± 0.50 1.00 ± 0.03 1.00 ± 0.05 1.00 ± 0.58
  Fenofibrate 0.1 μM 3.91 ± 0.20** 7.40 ± 0.82* 5.04 ± 0.03*** 6.52 ± 0.78** 0.97 ± 0.14
  Pioglitazone 0.1 μM 2.40 ± 0.75 3.94 ± 1.29 2.42 ± 0.11** 2.38 ± 1.00 1.93 ± 1.36
DAY   Osteocalcin Osteopontin PPARα PPARγ  
0   1.00 ± 0.23 1.00 ± 0.14 1.00 ± 0.51 1.00 ± 0.04  
4 Control 1.00 ± 0.34 1.00 ± 0.02 1.00 ± 0.05 1.00 ± 0.30  
  Fenofibrate 0.1 μM 0.54 ± 0.19 0.99 ± 0.14 2.51 ± 0.43* 0.86 ± 0.24  
  Pioglitazone 0.1 μM 1.38 ± 0.05 0.76 ± 0.02 1.76 ± 0.02* 1.06 ± 0.09  
8 Control 1.00 ± 0.20 1.00 ± 0.30 1.00 ± 0.21 1.00 ± 0.06  
  Fenofibrate 0.1 μM 0.54 ± 0.11 0.73 ± 0.04 2.83 ± 0.40* 0.93 ± 0.05  
  Pioglitazone 0.1 μM 0.67 ± 0.02 0.34 ± 0.02 0.98 ± 0.08 2.23 ± 0.36*  
12 Control 1.00 ± 0.10 1.00 ± 0.71 1.00 ± 0.04 1.00 ± 0.31  
  Fenofibrate 0.1 μM 2.34 ± 0.01** 2.38 ± 0.39 1.43 ± 0.23 1.20 ± 0.06  
  Pioglitazone 0.1 μM 1.89 ± 0.27 1.82 ± 2.32 No detectable expression 1.98 ± 0.61  
  1. PPAR = peroxisome proliferator activated receptor
  2. Data is in presented as mean ± SD, calculated from standard curves and related to housekeeping gene and normalized to controls. Data is from three biological replicates, *P < 0.05, **P < 0.01, ***P < 0.001 when compared to controls at same time (two-tailed student t-test).