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Table 5 Relative mRNA expression of selected genes in MC3T3-E1 cells

From: Different skeletal effects of the peroxisome proliferator activated receptor (PPAR)α agonist fenofibrate and the PPARγ agonist pioglitazone

DAY

 

Alcalic phosphatase (ALP)

Bone Sialoprotein

CD44

Collagen 1

Lipoprotein lipase (LPL)

0

 

1.00 ± 0.05

1.00 ± 0.44

1.00 ± 0.30

1.00 ± 0.10

No detectable expression

4

Control

1.00 ± 0.15

1.00 ± 0.18

1.00 ± 0.03

1.00 ± 0.24

1.00 ± 0.51

 

Fenofibrate 0.1 μM

0.62 ± 0.11

0.88 ± 0.23

1.05 ± 0.17

0.92 ± 0.18

1.12 ± 0.23

 

Pioglitazone 0.1 μM

1.06 ± 0.19

1.40 ± 0.62

1.52 ± 0.06

1.17 ± 0.19

0.90 ± 0.01

8

Control

1.00 ± 0.27

1.00 ± 0.54

1.00 ± 0.17

1.00 ± 0.03

1.00 ± 0.12

 

Fenofibrate 0.1 μM

1.47 ± 0.04

2.19 ± 0.24

1.09 ± 0.06

0.76 ± 0.0

0.81 ± 0.01

 

Pioglitazone 0.1 μM

1.40 ± 0.16

2.07 ± 1.45

0.81 ± 0.04

0.34 ± 0.04

1.00 ± 0.06

12

Control

1.00 ± 0.12

1.00 ± 0.50

1.00 ± 0.03

1.00 ± 0.05

1.00 ± 0.58

 

Fenofibrate 0.1 μM

3.91 ± 0.20**

7.40 ± 0.82*

5.04 ± 0.03***

6.52 ± 0.78**

0.97 ± 0.14

 

Pioglitazone 0.1 μM

2.40 ± 0.75

3.94 ± 1.29

2.42 ± 0.11**

2.38 ± 1.00

1.93 ± 1.36

DAY

 

Osteocalcin

Osteopontin

PPARα

PPARγ

 

0

 

1.00 ± 0.23

1.00 ± 0.14

1.00 ± 0.51

1.00 ± 0.04

 

4

Control

1.00 ± 0.34

1.00 ± 0.02

1.00 ± 0.05

1.00 ± 0.30

 
 

Fenofibrate 0.1 μM

0.54 ± 0.19

0.99 ± 0.14

2.51 ± 0.43*

0.86 ± 0.24

 
 

Pioglitazone 0.1 μM

1.38 ± 0.05

0.76 ± 0.02

1.76 ± 0.02*

1.06 ± 0.09

 

8

Control

1.00 ± 0.20

1.00 ± 0.30

1.00 ± 0.21

1.00 ± 0.06

 
 

Fenofibrate 0.1 μM

0.54 ± 0.11

0.73 ± 0.04

2.83 ± 0.40*

0.93 ± 0.05

 
 

Pioglitazone 0.1 μM

0.67 ± 0.02

0.34 ± 0.02

0.98 ± 0.08

2.23 ± 0.36*

 

12

Control

1.00 ± 0.10

1.00 ± 0.71

1.00 ± 0.04

1.00 ± 0.31

 
 

Fenofibrate 0.1 μM

2.34 ± 0.01**

2.38 ± 0.39

1.43 ± 0.23

1.20 ± 0.06

 
 

Pioglitazone 0.1 μM

1.89 ± 0.27

1.82 ± 2.32

No detectable expression

1.98 ± 0.61

 
  1. PPAR = peroxisome proliferator activated receptor
  2. Data is in presented as mean ± SD, calculated from standard curves and related to housekeeping gene and normalized to controls. Data is from three biological replicates, *P < 0.05, **P < 0.01, ***P < 0.001 when compared to controls at same time (two-tailed student t-test).