Table 1 Primers used in real-time PCR quantification
Gene | Primer Sequence | Specie | Size (bp) | Gene Bank Accession Number |
|---|---|---|---|---|
Alcalic phosphatase (ALP) | S 5'-AACCCAGACACAAGCATTCC-3' | Mouse | 151 | NM_007431.1 |
AS 5'-GAGAGCGAAGGGTCAGTCAG-3' | ||||
β-actin | S 5'-CTGGCTCCTAGCACCATGA-3' | Mouse | 73 | NM_031144.2 |
AS 5'-AGGCACCAATCCACACAGA-3' | ||||
Bone Sialoprotein | S 5'-GAAAATGGAGACGGCGATAG-3' | Mouse | 141 | NM_008318.1 |
AS 5'-ACCCGAGAGTGTGGAAAGTG-3' | ||||
CD44 | S 5'-CTTCCATCTTGACCCGTTGT-3' | Mouse | 175 | NM_009851.2 |
AS 5'-ACAGTGCTCCTGTCCCTGAT-3' | ||||
Collagen 1 | S 5'-AGAGCATGACCGATGGATTC-3' | Mouse | 177 | NM_007742.3 |
AS 5'-CCTTCTTGAGGTTGCCAGTC-3' | ||||
Lipoprotein lipase (LPL) | S 5'-GGATAAGCGACTCCTACTTC-3' | Mouse | 198 | NM_008509.1 |
AS 5'-AGCCAGACTTCTTCAGAGAC-3' | ||||
Osteocalcin | S 5'-CCGGGAGCAGTGTGAGCTTA-3' | Mouse | 81 | L24431.1 |
AS 5'-TAGATGCGTTTGTAGGCGGTC-3' | ||||
Osteopontin | S 5'-GACCACATGGACGACGATG-3' | Mouse | 498 | BC002113.1 |
AS 5'-TGGAACTTGCTTGACTATCGA-3' | ||||
PPARα | S 5'-TGAACAAAGACGGGATG-3' | Mouse | 106 | NM_011144.2 |
AS 5'-TCAAACTTGGGTTCCATGAT-3' | ||||
PPARγ | S 5'-CAGGCTTCCACTATGGAGTT-3' | Mouse | 105 | NM_011146.1 |
AS 5'-TCCGGCAGTTAAGATCACAC-3' |