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Table 1 Primers used in real-time PCR quantification

From: Different skeletal effects of the peroxisome proliferator activated receptor (PPAR)α agonist fenofibrate and the PPARγ agonist pioglitazone

Gene Primer Sequence Specie Size (bp) Gene Bank Accession Number
Alcalic phosphatase (ALP) S 5'-AACCCAGACACAAGCATTCC-3' Mouse 151 NM_007431.1
  AS 5'-GAGAGCGAAGGGTCAGTCAG-3'    
β-actin S 5'-CTGGCTCCTAGCACCATGA-3' Mouse 73 NM_031144.2
  AS 5'-AGGCACCAATCCACACAGA-3'    
Bone Sialoprotein S 5'-GAAAATGGAGACGGCGATAG-3' Mouse 141 NM_008318.1
  AS 5'-ACCCGAGAGTGTGGAAAGTG-3'    
CD44 S 5'-CTTCCATCTTGACCCGTTGT-3' Mouse 175 NM_009851.2
  AS 5'-ACAGTGCTCCTGTCCCTGAT-3'    
Collagen 1 S 5'-AGAGCATGACCGATGGATTC-3' Mouse 177 NM_007742.3
  AS 5'-CCTTCTTGAGGTTGCCAGTC-3'    
Lipoprotein lipase (LPL) S 5'-GGATAAGCGACTCCTACTTC-3' Mouse 198 NM_008509.1
  AS 5'-AGCCAGACTTCTTCAGAGAC-3'    
Osteocalcin S 5'-CCGGGAGCAGTGTGAGCTTA-3' Mouse 81 L24431.1
  AS 5'-TAGATGCGTTTGTAGGCGGTC-3'    
Osteopontin S 5'-GACCACATGGACGACGATG-3' Mouse 498 BC002113.1
  AS 5'-TGGAACTTGCTTGACTATCGA-3'    
PPARα S 5'-TGAACAAAGACGGGATG-3' Mouse 106 NM_011144.2
  AS 5'-TCAAACTTGGGTTCCATGAT-3'    
PPARγ S 5'-CAGGCTTCCACTATGGAGTT-3' Mouse 105 NM_011146.1
  AS 5'-TCCGGCAGTTAAGATCACAC-3'    
  1. PPAR = peroxisome proliferator activated receptor