Figure 2From: The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study Ucp1 is expressed in an insulin producing cell line: cDNAs prepared in the absence or presence of reverse transcriptase (RT) from total RNA isolated from MIN-6 and αTC1.6 cells, were used for PCR with the indicated primers. The lane containing 100 bp marker (M), and positions of Ucp1 and Ucp2 products are indicated. A band corresponding to Ucp1 (505 bp) can be clearly seen in the lane corresponding to RT+ MIN-6 cDNA. Expression of Ucp2 (608 bp) can be seen in both insulin and glucagon producing cell lines.Back to article page