Figure 4

DMA enables glucose to induce TDP in islets from both normal and diabetic mice: Insulin secretion in response to high glucose (16.7 mM) is shown, in groups of islets previously exposed to glucose with and without intracellular acidification produced by 40 μM DMA (denoted in red). DMA treatment enables glucose to induce TDP both in wild type controls (left) and each strain of type 2 diabetes (Right). G = mM glucose; n = 5; * = p < 0.001 and # = p < 0.05, compared with each corresponding untreated control. Values in the figure are expressed as fractional release (% of total insulin content released over the final 40-minute period in response to 16.7 mM glucose). The corresponding absolute amounts (ng/ml) for each condition from left to right are: 2.88 ± 0.57, 5.3 ± 0.76, 8.5 ± 0.93, 11.96 ± 2.14, 20.32 ± 6.12, 39.68 ± 5.69, 15.2 ± 3.23, 15.79 ± 2.59, and 19.79 ± 3.41.