Figure 3

Glucose induces TDP in mouse islets in the presence of DMA: Insulin secretion in response to 16.7 mM glucose, from freshly isolated islets that were previously exposed to different conditions as denoted in the X axis. All groups were first pre-incubated in basal glucose for one hour. During the next 40 minutes, islets were exposed to 2.8 or 16.7 mM glucose with and without 40 μM DMA. Following a subsequent rest period of 30 minutes in basal glucose, all groups were exposed to 16.7 mM glucose for one hour and samples collected for insulin assay. Insulin secretion is expressed as fractional release %, i.e. the percentage of the total insulin content released over the one-hour period of stimulation with 16.7 mM glucose. n = 8; G = mM glucose; * = p < 0.0001, compared with all other groups.