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Figure 2 | BMC Endocrine Disorders

Figure 2

From: Nutrient-stimulated insulin secretion in mouse islets is critically dependent on intracellular pH

Figure 2

Effect on islet pH i by different pH-altering methods: Islets loaded with SNARF5-AM were placed in basal KRBH, excited at 514 nm and emission fluorescence recorded at 580 and 630 nm. After a steady baseline was established, additions/substitutions were made as denoted in each figure. One representative recording for each condition is shown. A. 40 μM DMA: n = 16, average pH change = 0.5 ± 0.07; B. Removal of medium Cl-: n = 5, average pH change = 0.3 ± 0.06; C. Switching to a high pH (>8.3) medium: n= 6, average pH change = 0.24 ± 0.03; D. Switching to a Cl--free and high pH (>8.3) medium: n = 8, average pH change = 0.5 ± 0.06

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