Figure 1
From: Nutrient-stimulated insulin secretion in mouse islets is critically dependent on intracellular pH
Changes in emission fluorescence of SNARF5, in response to changes in intracellular pH: Islets loaded with SNARF5-AM (5 μM) were fixed at known intracellular pH values by placing them in KRBH solutions containing 100 mM K+ and 20 μM nigericin, adjusted to different pH values. Islets were excited at 514 nm, and emission fluorescence recorded at 580 and 630 nm. Emission fluorescence at 580 and 630 nm are denoted by red and green respectively. Top: Islets at intracellular pH 6, 7, and 8. As pH increases, red fluorescence decreases and green fluorescence increases. Bottom: Standard curve prepared using islets fixed at pHi values ranging from 5.5–9.0. n = 5–10 islets for each pH.