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Figure 1 | BMC Endocrine Disorders

Figure 1

From: Testicular histological and immunohistochemical aspects in a post-pubertal patient with 5 alpha-reductase type 2 deficiency: case report and review of the literature in a perspective of evaluation of potential fertility of these patients

Figure 1

Histological characteristics and immunohistochemical detection for AR, AMH and 3βHSD expression, in testicular paraffin-embedded sections obtained from a 29 year old human adult with normal testicular structures (Figure  1 : panels A, D, G, J), a 17 year-old teenager with 5 α-R2 deficiency (Figure  1 : panels B, E, H, K) and a 18 year-old CAIS teenager (Figure  1 : panels C, F, I, L). Panels A, B, C- histology of testicular samples on haematoxylin-eosin staining. Panel A shows complete spermatogenesis in a seminiferous tubule section of the normal adult testis; Panel B shows heterogeneous seminiferous tubules (ST), in the 5 α-R2 deficiency, such as tubules presenting large diameters and lumina(white arrows) and other tubules with small diameter lacking lumina (thick black arrow); Panel C, shows, in a CAIS patient, used for comparison, a homogeneous pattern, with small diameter, immature STs. In this CAIS patient, STs are delineated by a thickened basal membrane (thick black arrow). In one ST, Sertoli cells with an oncocytic transformation of the cytoplasm are indicated (white arrow).The interstitial compartment contains an area of Leydig cell hyperplasia (thin black arrow). Panels D, E, F- show AR immune detection in testicular sections obtained from the three subjects. AR is immunodetected in Sertoli cells, Leydig cells and peritubular myoid cells. Panel F evidentiates positive AR immunostaning in ST of the CAIS patient's testicular sample. Panels G, H, I- show AMH immune detection in STs of the testicular sections obtained from the three subjects. Panels J, K, L- show 3βHSD immune detection in Leydig cells of the testicular sections obtained from the three subjects. Scale bars -50 μm.

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