Figure 6
25(OH)D by LC-MS/MS - WCMC dataset. A. 25(OH)D immunoassay results versus LC-MS/MS total 25(OH)D results in cases where both tests were ordered by clinicians on the same specimen. Deming regression (red dashed line) shows a slope of 0.958 and a Y-intercept of 4.7 ng/ml. B. Corresponding fractionated 25(OH)D2 and 25(OH)D3 results from specimens ordered for 25(OH)D testing by LC-MS/MS from WCMC. See Results section for n-values. Many data points are obscured due to overlapping position. Both X- and Y-axes are cropped at 100 ng/ml to improve visibility. Red dots (many overlapping) = unquantifiable 25(OH)D2, quantifiable 25(OH)D3. Green dots = quantifiable 25(OH)D2, unquantifiable 25(OH)D3. Black dot (overlapping) = unquantifiable 25(OH)D2 and unquantifiable 25(OH)D3. Blue dots = quantifiable 25(OH)D2 and quantifiable 25(OH)D3. C,D. Average 25(OH)D2 (gray line) and average 25(OH)D3 (magenta line) results age and sex (C, females; D, males) at WCMC. Variability may be due to comparatively small n-values as shown by specimen counts in each 1 yr bin (E, females; F, males). G,H. Area plots show the reference interval distributions for all ages (1–90 yrs; 1 yr bins) in females (G) and males (H) at WCMC. Figure legend (color) for area plots is identical to Figure 5A.