Table 2 VDR polymorphisms and PCR-RFLP information
SNP | Localization | Base change | Nomenclature | Forward primer/reverse primer | Amplicone | Restriction site | Digestion fragments | Genotype | Restriction enzyme | Reference |
|---|---|---|---|---|---|---|---|---|---|---|
FokI | Exon 2 | C/T | FF Ff ff | 5'-AgC Tgg CCC Tgg CAC TgA CTC TgC TCT-3' 5'-ATg gAA ACA CCT TgC TTC TTC TCC CTC-3' | 265 | CC CT TT | 207-60 | mut ht wt | FokI | [45] |
BsmI | Intron 8 | G/A | BB Bb bb | 5'-ggC AAC CTg AAg ggA gAC gTA-3' 5'-CTC TTT ggA CCT CAT CAC CgA C-3' | 461 | AA GA GG | 258-253 | mut ht wt | Mva 1269I | [46] |
TaqI | Exon 9 | T/C | TT Tt tt | 5'-CAg AgC ATg gAC Agg gAg CAA g-3' 5'-gCA ACT CCT CAT ggC TgA ggT CTC A-3' | 740 | TT TC CC | 495-245-290-205 | wt ht mut | TaqI | [45] |
ApaI | Intron 8 | G/T | AA Aa aa | 5'-CAg AgC ATg gAC Agg gAg CAA g-3' 5'-gCA ACT CCT CAT ggC TgA ggT CTC A-3' | 740 | TT GT GG | 515-225 | mut ht wt | Bsp120I | [45] |
Cdx-2 | Intron 1e | G/A | CC Cc cc | 5'-CAg CAT gCC TgT CCT CAg C-3' 5'-CCA gTA CTg CCA gCT CCC-3' | 250 | AA GA GG | Â | mut ht wt | Bpu10I | [47] |