Table 1 Primer Pairs Used for Quantitative Reverse Transcriptase Polymerase Chain Reaction Assays

Insulin

For

TTC TAC ACA CCC AAG TCC CGT C

145

135

Insulin

Rev

ATC CAC AAT GCC ACG CTT CTG C

279

Insulin Receptor

For

TGA CAA TGA GGA ATG TGG GGA C

875

129

Insulin Receptor

Rev

GGG CAA ACT TTC TGA CAA TGA CTG

1003

IGF-I

For

GAC CAA GGG GCT TTT ACT TCA AC

65

127

IGF-I

Rev

TTT GTA GGC TTC AGC GGA GCA C

191

IGF-I Receptor

For

GAA GTC TGC GGT GGT GAT AAA GG

2138

113

IGF-I Receptor

Rev

TCT GGG CAC AAA GAT GGA GTT G

2250

IGF-II

For

CCA AGA AGA AAG GAA GGG GAC C

763

95

IGF-II

Rev

GGC GGC TAT TGT TGT TCA CAG C

857

IGF-II Receptor

For

TTG CTA TTG ACC TTA GTC CCT TGG

1066

91

IGF-II Receptor

Rev

AGA GTG AGA CCT TTG TGT CCC CAC

1156

IRS-1

For

GAT ACC GAT GGC TTC TCA GAC G

604

134

IRS-1

Rev

TCG TTC TCA TAA TAC TCC AGG CG

737

IRS-2

For

CAA CAT TGA CTT TGG TGA AGG GG

255

109

IRS-2

Rev

TGA AGC AGG ACT ACT GGC TGA GAG

263

IRS-4

For

ACC TGA AGA TAA GGG GTC GTC TGC

2409

132

IRS-4

Rev

TGT GTG GGG TTT AGT GGT CTG G

2540

ChAT

For

TCA CAG ATG CGT TTC ACA ACT ACC

478

106

ChAT

Rev

TGG GAC ACA ACA GCA ACC TTG

583

AChE

For

TTC TCC CAC ACC TGT CCT CAT C

420

123

AChE

Rev

TTC ATA GAT ACC AAC ACG GTT CCC

542

APP

For

GCA GAA TGG AAA ATG GGA GTC AG

278

199

APP

Rev

AAT CAC GAT GTG GGT GTG CGT C

476

Tau

For

CGC CAG GAG TTT GAC ACA ATG

244

65

Tau

Rev

CCT TCT TGG TCT TGG AGC ATA GTG

308

SPTLC-1

For

CTAACCTTGGGCAAATCGAA

2581

96

SPTLC-1

Rev

TGAGCAGGGAGAAGGGACTA

2676

SPTLC-2

For

GGA CAG TGT GTG GCC TTT CT

1823

50

SPTLC-2

Rev

TCA CTG AAG TGT GGC TCC TG

1872

CERS-1

For

TGC GTG AAC TGG AAG ACT TG

947

98

CERS-1

Rev

CTT CAC CAG GCC ATT CCT TA

1044

CERS-2

For

CTC TGC TTC TCC TGG TTT GC

698

82

CERS-2

Rev

CCA GCA GGT AGT CGG AAG AG

779

CERS-4

For

CGA GGC AGT TTC TGA AGG TC

1240

72

CERS-4

Rev

CCA TTG GTA ATG GCT GCT CT

1311

CERS-5

For

GAC AGT CCC ATC CTC TGC AT

1254

92

CERS-5

Rev

GAG GTT GTT CGT GTG TGT GG

1345

UGCG

For

GAT GCT TGC TGT TCA CTC CA

2682

67

UGCG

Rev

GCT GAG ATG GAA GCC ATA GG

2748

SMPD-1

For

CAG TTC TTT GGC CAC ACT CA

1443

65

SMPD-1

Rev

CGG CTC AGA GTT TCC TCA TC

1507

SMPD-3

For

TCT GCT GCC AAT GTT GTC TC

2704

98

SMPD-3

Rev

CCG AGC AAG GAG TCT AGG TG

2801