Possible modulatory effect of endogenous islet catecholamines on insulin secretion

Abstract

Background

The possible participation of endogenous islet catecholamines (CAs) in the control of insulin secretion was tested.

Methods

Glucose-induced insulin secretion was measured in the presence of 3-Iodo-L-Tyrosine (MIT), a specific inhibitor of tyrosine-hydroxylase activity, in fresh and precultured islets isolated from normal rats. Incubated islets were also used to measure CAs release in the presence of low and high glucose, and the effect of α2-(yohimbine [Y] and idazoxan [I]) and α1-adrenergic antagonists (prazosin [P] and terazosin [T]) upon insulin secretion elicited by high glucose.

Results

Fresh islets incubated with 16.7 mM glucose released significantly more insulin in the presence of 1 μM MIT (6.66 ± 0.39 vs 5.01 ± 0.43 ng/islet/h, p < 0.02), but did not affect significantly the insulin response to low glucose. A similar enhancing effect of MIT upon insulin secretion was obtained using precultured islets devoid of neural cells, but absolute values were lower than those from fresh islets, suggesting that MIT inhibits islet rather than neural tyrosine hydroxylase. CAs concentration in the incubation media of fresh isolated islets was significantly higher in the presence of 16.7 than 3.3 mM glucose: dopamine 1.67 ± 0.13 vs 0.69 ± 0.13 pg/islet/h, p < 0.001, and noradrenaline 1.25 ± 0.17 vs 0.49 ± 0.04 pg/islet/h, p < 0.02. Y and I enhanced the release of insulin elicited by 16.7 mM glucose while P and T decreased such secretion.

Conclusion

Our results suggest that islet-originated CAs directly modulate insulin release in a paracrine manner.

Background

Insulin secretion in response to glucose is modulated by neural [1], hormonal and paracrine [2] factors that bestow great precision to the stimulus: secretion coupling process. Catecholamines (CAs) contribute to this mechanism by exerting a direct and dual effect on the B-cell to induce either inhibition or stimulation of insulin secretion through their interaction with α₂ or α₁ and β₂ adrenergic receptors, respectively [3, 4]. Since islet B-cells have more α₂ than α₁ and β₂ adrenergic receptors, physiological concentrations of CAs will bind mainly to the α₂ population receptors and thus inhibit insulin secretion [5–8].

Although the effect of the sympathetic nervous system (SNS) and of circulating CAs on islet physiology has been studied in both normal and pathological states, little is known about the possible participation of endogenously-generated CAs in the control of islet function. Islet cells have been shown to contain enzymes involved both in the synthesis of CAs – tyrosine hydroxylase (TH) [9, 10] and dihydroxyphenylalanine (DOPA) decarboxylase [11, 12] – and in their inactivation – monoamine oxidase [13, 14].

We have recently measured TH activity in normal rat isolated islets, showing an increase in the enzyme's activity, and a decrease of CAs content and insulin release in rats fed only with carbohydrates [15]. In these experiments, comparable TH activity values were measured in islets isolated from either control or solarectomized rats, thus suggesting that the enzyme activity was of islet rather than neural origin.

In order to gain evidence about the possible modulatory role of CAs of endogenous islet origin as paracrine hormonal regulators of insulin secretion, we have currently studied: a) The effect of 3-Iodo-L-Tyrosine (MIT) – a selective drug usually used for the acute inhibition of TH in neuroendocrine tissues [16] – on the insulin secretory response of either fresh or precultured isolated islets to glucose, and b) The effect of specific α₂- or α₁-adrenergic receptor antagonists upon glucose-induced insulin secretion in isolated islets, and c) The release of endogenous CAs by endocrine islet cells.

Methods

Chemicals and drugs

Collagenase was obtained from Serva Feinbiochemica, (Heidelberg, Germany), while bovine serum albumin, fraction V and other reagents of the purest available grade were purchased from Sigma Chemical Co. (St. Louis, USA).

Animals and islet isolation

Male Wistar rats (180–200 g) were used as a source of islets. They were fed ad libitum and kept under conditions of controlled temperature and lighting (12 h light and 12 h dark). After enzymatic digestion with collagenase, pancreases were repeatedly washed and the islets rapidly hand-picked with siliconized glass pipettes under a dissecting microscope [17].

Islet culture

Isolated islets were cultured at 37°C in a humidified atmosphere for 24 h in plastic Petri dishes (NUNC) with RPMI 1640 supplemented with Hepes (20 mM), CO₃HNa (4.1 mM), penicillin, streptomycin (100.000 U/I and 100 mg/l, respectively), 10% newbom calf serum [18], and 3 mM glucose. The pH was adjusted to 7.4. After the first 24-h period, the culture medium was replaced by fresh medium with the same glucose concentration; afterwards, the medium was changed every second day until accomplishing the total one-week culture period.

Islet incubation

Groups of 5 fresh or precultured isolated islets were incubated for 30 min at 37°C in 600 μl Krebs-Ringer-bicarbonate buffer with 1% (w/v) bovine-serum albumin, Trasylol™ (400 IU/ml), and either 3.3 or 16.7 mM glucose, with or without MIT (1 μM) [19]. The medium had been previously gassed with a mixture of 5% CO₂:95% O₂ (v/v) to adjust the pH to 7.4.

Separately, groups of 5 fresh isolated islets were incubated for 60 min in the presence of α₂-(yohimbine [Y, 0.1 and 1 μM] or idazoxan [I, 1 μM]) and α₁- (prazosin [P, 0.1 and 0.5 μM] or terazosin [T, 0.1 and 0.5 μM]) adrenergic antagonists. In all cases, aliquots from the medium were obtained at the end of the incubation period and kept frozen until insulin determination by radioimmunoassay [20].

CAs quantitation

Another group of islets was incubated for 30 min in the presence of 3.3 and 16.7 mM glucose. Following that incubation period, CAs were measured in the incubation media after partial purification by batch alumina extraction, separated by reverse-phase high-pressure liquid chromatography on a 4.6 X 250-mm Zorbax Rp C18 column (DuPont), and quantified amperometrically with a triple-electrode system (ESA, Bedford, NA) by measuring the current produced upon exposure of the column effluent to first oxidizing and then reducing potentials in series [21].

Data analysis

For the statistical evaluation of the data, we employed both variance analysis and the paired Student t-test.

Results

The addition of 1 μM MIT to the incubation medium of fresh isolated islets enhanced significantly the release of insulin elicited by 16.7 mM glucose (Fig. 1, upper pannel; p < 0.02). Such effect was not observed in the presence of a low glucose concentration. Similarly, MIT enhanced significantly the release of insulin elicited by high glucose in precultured islets (Fig. 1, lower pannel; p < 0.05). In this case, however, the absolute insulin concentration values measured in the incubation media were lower than those measured in the experiments performed with fresh islets.

Figure 1

Effect of MIT upon insulin secretion elicited by 16.7 mM glucose in fresh (upper pannel) and precultured (lower pannel) isolated islets of Langerhans. Each bar represents the mean ± SEM and refers to 3 separate experiments and 12 individual determinations. P values were <0.02 and <0.05 for fresh and precultured islets, respectively.

The assessment of CAs in the incubation media from freshly isolated islets showed a glucose-induced increase in the concentration of dopamine and noradenaline (3.3 vs 16.7 mM glucose): dopamine 0.69 ± 0.13 (n = 4) vs 1.67 ± 0.13 (n = 6) pg/islet/h; p < 0.001, and noradrenaline 0.49 ± 0.04 (n = 3) vs 1.25 ± 0.17 (n = 3) pg/islet/h; p < 0.02.

Addition of selective α₂- and α₁- CA receptor antagonists to the medium significantly affected the insulin released by islets incubated with glucose: α₂-antagonists Y (0.1 and 1 μM) and I (1 μM) enhanced significantly the release of insulin in response to 16.7 mM glucose (p < 0.05,0.001, and 0.001, respectively; Fig. 2, upper pannel), while α₁-antagonists P and T (0.1 and 0.5 μM) decreased it significantly (P, p < 0.02 and 0.005, and T, p < 0.001, 0.001, respectively; Fig. 2, lower pannel). Excepting I, the effect of the antagonists was dose-dependent: insulin release increased 82, 171 and 895 % in the presence of 0.1 and 1 μM Y and 1 μM I, respectively, while it decreased 40 and 49% with 0.1 and 0.5 μM P, and 51 and 45% in the presence of T at the same concentrations.

Figure 2

Effect of α₂- (Y and I, upper pannel) and α₁- (P and T, lower pannel) adrenergic antagonists upon insulin secretion in isolated islets of Langerhans incubated in the presence of 16.7 mM glucose. Each bar represents the mean ± SEM and refers to 3 separate experiments and 10 individual determinations. The concentrations of Y, I, P, and T are shown under each bar. P values: a vs b, p < 0.05; a vs c and d, p < 0.001; e vs f, p < 0.02; e vs g, p < 0.005; e vs h and i, p < 0.001.

Discussion

In our experiments, MIT addition to the incubation medium induced a significant increase in glucose-induced insulin release, either from fresh or precultured isolated islets. Incubation of different tissues with MIT, for even shorter periods than the one we used, blocks significantly the activity of TH, the first limiting step enzyme in CA biosynthesis [16]. The presence of immunochemically demonstrable TH in α and B islet cells has been reported in fetal-mouse pancreases [9], and in the B-cells of several adult rodents (Sprague Dawley rats, two strains of mice, and pigmented guinea pigs) [10]. Further, we have measured TH activity in the endocrine pancreas of normal adult rats, recording comparable TH activity values in islets isolated from either control or solarectomized rats (330 ± 40 vs 300 ± 80 pmol/mg protein/h), indicating that the TH activity measured was of islet rather than neural origin [15]. We could then assume that as in other tissues, TH inhibition by MIT decreases CA production rate in the islets, and consequently its availability [22]. Following this reasoning, it could be argued that, at least in our model, CAs synthesis (or availability) participates in the regulation of insulin secretion.

Our cultured islets would be devoid of adrenergic innervation since sympathetic neurons maintained in a culture medium with a Ca²⁺ concentration above 2 mM (condition employed in our islet culture) do not survive more than 6–8 days [23]. Since the blocking effect of MIT was also observed using cultured islets, we can assume that islet rather than neural CAs biosynthesis is involved in this modulatory process of insulin secretion. We cannot however completely exclude the possibility that MIT could affect insulin secretion either directly or by modifying other processes than CAs biosynthesis.

There is considerable evidence in the literature for the possible presence of CAs biosynthesis in islet tissue: a) We have recently reported the immunocytochemical identification of DOPA decarboxylase in glucagon-containing cells of normal adult rat islets [12]; b) Oomori et al. have reported the presence of dopamine beta-hydroxylase immunoreactivity in rat pancreatic cells which – according to their features – were neither neural nor endocrine cells, and suggested that these cells may release norepinephrine (NE) to adequate stimuli [24]; c) Lundquist et al. detected dopamine in islets obtained from chemically-sympathectomized mice by means of high-performance liquid chromatography [25]; and d) Falck and Hellman showed that B-cells from guinea-pig islets stored monoamines [26, 27] despite their failure to observe sympathetic nerves in the endocrine pancreas of those animals.

CAs inhibit glucose-induced insulin secretion by interacting with α₂-adrenoceptors in B-cells, but could stimulate insulin secretion by two differents actions: activation of B-cell α₁ and β₂ adrenoceptors [28], and a direct action on α-cells, mediated by α₂ and β₂ adrenoceptors, which stimulate glucagon secretion [29, 30]. We have currently observed that high glucose induced an increase in the release of CAs from normal rat isolated islets to the incubation media. We have also found that different α₂- and α₁-adrenergic antagonists added to the incubation media significantly enhanced and decreased, respectively, the secretion of insulin elicited by glucose in a dose-dependent manner. The adrenoceptor blockade by Y increased the secretion of insulin by 82 and 172% while I enhanced such secretion by 895%. Conversely, P and T diminished insulin secretion by 40–49% and 51–45%, respectively, with the doses currently employed. As shown by other authors, the uneven effect of adrenergic antagonists could be ascribed to the relative abundance or activity of α₂-adrenoceptors in B-cells, as compared with α₁- and β₂-adrenoceptors [5–8]. On the other hand, Schuit et al. showed that P was ineffective in both a and B-cells [31]; such discrepancy with our results can be attributed to the method used in each case.

Since we did not add CAs to the incubation media, it could be assumed that the effect of the antagonists was due to an interference with the action of CAs, elaborated and co-released with insulin by endocrine islet cells. The results presented here as well our previous report on the glucose-induced release of CAs by isolated rat islets [32] support this assumption. On account of these results, therefore, we postulate that islet-originated CAs could directly modulate the release of insulin in a paracrine hormonal manner.